Not known Factual Statements About thr77
Not known Factual Statements About thr77
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important function in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This information outlines even more feasible optimization of mobile and immunoassay parameters to make certain the absolute best benefits are...
Antibodies sure to the blocking peptide no more bind to your epitope to the goal protein. By comparing the staining with the blocked antibody versus the antibody on your own, one can see which staining is specific.
Target Details Cyclin E belongs towards the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation styles which add to the temporal coordination of each mitotic party. Cyclin E types a posh with and capabilities being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S phase boundary which is degraded as cells progress through S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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